Quantprime Tool name | Quantprime |
---|
URL | http://www.quantprime.de/ |
---|
Important features | Designs Junction primers, quantitative real time PCR primers [SYBR Green real-time qPCR] and endpoint semi-quantitative-PCR primers [with or without splice variant hits]. |
---|
Citations | |
---|
Year of publication | Not available |
---|
Rank by usage frequency | 100 |
---|
Comments | |
---|
Function | Junction primers, Real time PCR, Primer specificity checking |
---|
Category | Online/downloadable, Free for academic |
---|
Features | 1. Allows for splice variants checking (primer pairs can be either designed to target all splice variants or only one transcript form) and user can select the organism and its transcriptome for which the primers has to be designed.
2. In addition primer pairs can be classified as bad, acceptable, good and very good by using color codes, user can store primer data in public server for one month, can add multiple transcripts as input for primer designing.
3. Under customization users can allow the tool to design primers with or without SNP.
4. Options to specify the primer length, primer Tm, GC%, amplicon Tm, product size, salt concentration etc. |
---|
Description | |
---|
| |