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A comparative account of primer designing resources, feature-validations and application-based categorizations

Quantprime

Tool nameQuantprime
URLhttp://www.quantprime.de/
Important featuresDesigns Junction primers, quantitative real time PCR primers [SYBR Green real-time qPCR] and endpoint semi-quantitative-PCR primers [with or without splice variant hits].
Citations
Year of publicationNot available
Rank by usage frequency100
Comments
FunctionJunction primers, Real time PCR, Primer specificity checking
CategoryOnline/downloadable, Free for academic
Features1. Allows for splice variants checking (primer pairs can be either designed to target all splice variants or only one transcript form) and user can select the organism and its transcriptome for which the primers has to be designed. 2. In addition primer pairs can be classified as bad, acceptable, good and very good by using color codes, user can store primer data in public server for one month, can add multiple transcripts as input for primer designing. 3. Under customization users can allow the tool to design primers with or without SNP. 4. Options to specify the primer length, primer Tm, GC%, amplicon Tm, product size, salt concentration etc.
Description

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